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rabbit polyclonal antibody against p nfκb ser536 (Bioss)

Name: rabbit polyclonal antibody against p nfκb ser536
Brand: Bioss
Rating: 94 (27 reviews)

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Bioss rabbit polyclonal antibody against p nfκb ser536
Rabbit Polyclonal Antibody Against P Nfκb Ser536, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit polyclonal antibody against p nfκb ser536/product/Bioss
Average 94 stars, based on 27 article reviews

rabbit polyclonal antibody against p nfκb ser536 - by Bioz Stars, 2026-02

94/100 stars

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( A ) Western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO human endometrial stromal cell (HESC) treated with differential culture medium for 4 days, nuclear translocation of p65 and p50 were higher in GNAQ-KO decidual cells than in control. GAPDH and histone 3 (H3) were used as the loading controls. ( B ) Immunofluorescence staining of p65 in CON and KO stromal cells and treated with differential culture medium for 4 days, nuclear translocation p65 were higher in GNAQ-KO decidual cells than in control. Scale bars, 100 μm. ( C ) Western blot analysis of p-p65 expression in whole-cell lysates of CON and KO cells treated with differential culture medium for 2, 4, and 6 days, phosphorylation of the nuclear factor (NF)-κB subunit p65 were higher in GNAQ-KO decidual cells than in control. GAPDH was used as the loading controls. ( D, E ) qRT-PCR analysis of IL-11 and IL-1β mRNA expression levels in CON and KO HESC treated as in ( C ) ( n = 5). ( F ) Western blot analysis of p-STAT3, STAT3, and IL-1β expression in whole-cell lysates of CON and KO HESC treated as in ( C ). β-Tubulin was used as the loading control. ( G, H, J, K ) qRT-PCR analysis of IL-11 , IL-1β , PRL , and IGFBP-1 mRNA after treatment with NF-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM), in CON and KO HESC treated with differential culture medium for 4 days ( n = 3). ( I, L ) Western blot analysis of p-STAT3, IL-1β, and IGFBP-1 expression in whole-cell lysates of differentiated CON and KO HESC treated as in ( G ). GAPDH was used as the loading control. Representative data are shown from three to five independent experiments. ( D and E ) were analyzed with unpaired Student’s t -test. *p < 0.05 . ( G, H, J, and K ) were calculated with one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison tests. *p < 0.05. Figure 2—source data 1. Original blot images of .

Journal: eLife

Article Title: Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

doi: 10.7554/eLife.83083

Figure Lengend Snippet: ( A ) Western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO human endometrial stromal cell (HESC) treated with differential culture medium for 4 days, nuclear translocation of p65 and p50 were higher in GNAQ-KO decidual cells than in control. GAPDH and histone 3 (H3) were used as the loading controls. ( B ) Immunofluorescence staining of p65 in CON and KO stromal cells and treated with differential culture medium for 4 days, nuclear translocation p65 were higher in GNAQ-KO decidual cells than in control. Scale bars, 100 μm. ( C ) Western blot analysis of p-p65 expression in whole-cell lysates of CON and KO cells treated with differential culture medium for 2, 4, and 6 days, phosphorylation of the nuclear factor (NF)-κB subunit p65 were higher in GNAQ-KO decidual cells than in control. GAPDH was used as the loading controls. ( D, E ) qRT-PCR analysis of IL-11 and IL-1β mRNA expression levels in CON and KO HESC treated as in ( C ) ( n = 5). ( F ) Western blot analysis of p-STAT3, STAT3, and IL-1β expression in whole-cell lysates of CON and KO HESC treated as in ( C ). β-Tubulin was used as the loading control. ( G, H, J, K ) qRT-PCR analysis of IL-11 , IL-1β , PRL , and IGFBP-1 mRNA after treatment with NF-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM), in CON and KO HESC treated with differential culture medium for 4 days ( n = 3). ( I, L ) Western blot analysis of p-STAT3, IL-1β, and IGFBP-1 expression in whole-cell lysates of differentiated CON and KO HESC treated as in ( G ). GAPDH was used as the loading control. Representative data are shown from three to five independent experiments. ( D and E ) were analyzed with unpaired Student’s t -test. *p < 0.05 . ( G, H, J, and K ) were calculated with one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison tests. *p < 0.05. Figure 2—source data 1. Original blot images of .

Article Snippet: The membrane was blocked in 5% skimmed milk (BD) and probed with rabbit polyclonal antibody against Gαq, rabbit monoclonal antibody against IGFBP-1 (Abcam, Cat# ab181141, 1:1000), mouse monoclonal antibody against IL-1β (Origene Inc, Cat# TA506443, 1:1000), rabbit polyclonal antibody against p65 (Abcam, Cat# ab32536, 1:1000), rabbit polyclonal antibody against p50 (Abcam Cat# ab32360, 1:1000), p-STAT3 (Abcam Cat# ab76315, 1:1000), STAT3 (Abcam Cat# ab68153, 1:1000), rabbit polyclonal antibody against IκBa (Abcam Cat# ab32518, 1:1000), rabbit polyclonal antibody against p-NFκB p65(Ser536) (Bioss, Cat# bs-0982R, 1:1000), mouse monoclonal antibody against p-IκBа (Ser32) (Bioss, Cat# bsm-52169R, 1:1000), rabbit monoclonal antibody against p-PKA C(Thr197) (Cell Signaling Technology, Cat# 5661, 1:1000), rabbit monoclonal antibody against PKA C-а (Cell Signaling Technology, Cat# 5842, 1:1000), rabbit polyclonal antibody against p-PKCа/βǁ (Thr638/641) (Cell Signaling Technology, Cat# 9375, 1:1000), rabbit polyclonal antibody against PKCа (Cell Signaling Technology, Cat# 2056, 1:1000) and p-PKC Antibody Sampler Kit (Cell Signaling Technology, Cat# 9921, 1:1000), mouse monoclonal antibody against HDAC5 (Cell Signaling Technology, Cat# 98329, 1:1000), and rabbit polyclonal antibody against p-HDAC5 (ABclonal, Cat# AP0202, 1:1000).

Techniques: Western Blot, Expressing, Translocation Assay, Control, Immunofluorescence, Staining, Quantitative RT-PCR, Comparison

( A ) Immunofluorescence staining of p50 in CON and KO differentiated stromal cells and treated with differential culture medium for 4 days, nuclear translocation of the p50 were higher in GNAQ-KO decidual cells than in control. Scale bars, 100 μm. ( B ) Immunofluorescence staining of p65 and p50 after treatment with nuclear factor (NF)-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM), in differentiated CON and KO human endometrial stromal cell (HESC) treated with differential culture medium for 4 days, PDTC blocked p65 and p50 translocate to the nucleus. Scale bars, 100 μm.

Journal: eLife

Article Title: Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

doi: 10.7554/eLife.83083

Figure Lengend Snippet: ( A ) Immunofluorescence staining of p50 in CON and KO differentiated stromal cells and treated with differential culture medium for 4 days, nuclear translocation of the p50 were higher in GNAQ-KO decidual cells than in control. Scale bars, 100 μm. ( B ) Immunofluorescence staining of p65 and p50 after treatment with nuclear factor (NF)-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM), in differentiated CON and KO human endometrial stromal cell (HESC) treated with differential culture medium for 4 days, PDTC blocked p65 and p50 translocate to the nucleus. Scale bars, 100 μm.

Techniques: Immunofluorescence, Staining, Translocation Assay, Control

( A, B ) Relative of western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO. The values are normalized to the GAPDH or H3 expression level and indicated as the mean ± standard error of the mean (SEM) of three independent experiments ( n = 3, *p < 0.05, versus controls). ( C–E ) Relative of western blot analysis of p-p65, p-STAT3, and IL-1β expression in whole-cell lysates of CON and KO cells. The values are normalized to the GAPDH or β-Tubulin expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls). ( F-H ) Relative of western blot analysis of p-STAT3, IL-1β, and IGFBP-1 expression in whole-cell lysates of differentiated CON and KO human endometrial stromal cell (HESC) treated with nuclear factor (NF)-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM). The values are normalized to the GAPDH or β-Tubulin expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls).

Journal: eLife

Article Title: Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

doi: 10.7554/eLife.83083

Figure Lengend Snippet: ( A, B ) Relative of western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO. The values are normalized to the GAPDH or H3 expression level and indicated as the mean ± standard error of the mean (SEM) of three independent experiments ( n = 3, *p < 0.05, versus controls). ( C–E ) Relative of western blot analysis of p-p65, p-STAT3, and IL-1β expression in whole-cell lysates of CON and KO cells. The values are normalized to the GAPDH or β-Tubulin expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls). ( F-H ) Relative of western blot analysis of p-STAT3, IL-1β, and IGFBP-1 expression in whole-cell lysates of differentiated CON and KO human endometrial stromal cell (HESC) treated with nuclear factor (NF)-κB inhibitor, pyrrolidinedithiocarbamate ammonium (PDTC, 10 μM). The values are normalized to the GAPDH or β-Tubulin expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls).

Techniques: Western Blot, Expressing

( A ) qRT-PCR analysis of NFκBIA mRNA expression in proliferative (D0) and decidualized human endometrial stromal cell (HESC) treated with differential culture medium for 2, 4, and 6 days ( n = 3). ( B ) Western blot analysis IκBα and p-IκBα expression in whole-cell lysates of proliferative (D0) and decidualized HESC treated as in ( A ). GAPDH was used as the loading control. ( C ) Immunofluorescence staining of p65 and p50 in CON and KO stromal cells and treated with lentivirus transfected with empty vector or NFκBIA overexpression lentivirus for 3 days. Scale bars, 100 μm. ( D ) Western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of decidualized HESC treated with lentivirus transfected with empty vector or NFκBIA overexpression lentivirus for 3 days. GAPDH and H3 were used as the loading controls. ( E ) qRT-PCR analysis of IGFBP-1 mRNA expression levels in CON and KO HESC treated as in ( C ) ( n = 3). ( F ) Western blot analysis of the IGFBP-1, p-STAT3, and STAT3 expression in whole-cell lysates of decidualized HESC treated as in ( C ). GAPDH was used as the loading controls. Representative data are shown from three independent experiments. ( A ) was analyzed with unpaired Student’s t -test. ( E ) was calculated with one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison tests. *p < 0.05. Figure 3—source data 1. Original blot images of .

Journal: eLife

Article Title: Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

doi: 10.7554/eLife.83083

Figure Lengend Snippet: ( A ) qRT-PCR analysis of NFκBIA mRNA expression in proliferative (D0) and decidualized human endometrial stromal cell (HESC) treated with differential culture medium for 2, 4, and 6 days ( n = 3). ( B ) Western blot analysis IκBα and p-IκBα expression in whole-cell lysates of proliferative (D0) and decidualized HESC treated as in ( A ). GAPDH was used as the loading control. ( C ) Immunofluorescence staining of p65 and p50 in CON and KO stromal cells and treated with lentivirus transfected with empty vector or NFκBIA overexpression lentivirus for 3 days. Scale bars, 100 μm. ( D ) Western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of decidualized HESC treated with lentivirus transfected with empty vector or NFκBIA overexpression lentivirus for 3 days. GAPDH and H3 were used as the loading controls. ( E ) qRT-PCR analysis of IGFBP-1 mRNA expression levels in CON and KO HESC treated as in ( C ) ( n = 3). ( F ) Western blot analysis of the IGFBP-1, p-STAT3, and STAT3 expression in whole-cell lysates of decidualized HESC treated as in ( C ). GAPDH was used as the loading controls. Representative data are shown from three independent experiments. ( A ) was analyzed with unpaired Student’s t -test. ( E ) was calculated with one-way analysis of variance (ANOVA) with Bonferroni’s multiple comparison tests. *p < 0.05. Figure 3—source data 1. Original blot images of .

Techniques: Quantitative RT-PCR, Expressing, Western Blot, Control, Immunofluorescence, Staining, Transfection, Plasmid Preparation, Over Expression, Comparison

( A, B ) Relative of western blot analysis of IκBα and p-IκBα expression in whole-cell lysates of CON and KO cells. The values are normalized to the GAPDH expression level and indicated as the mean ± standard error of the mean (SEM) of three independent experiments ( n = 3, *p < 0.05, versus controls). ( C, D ) Relative of western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO treated with lentivirus transfected with empty vector or NF κ BIA overexpression lentivirus for 3 days. The values are normalized to the GAPDH or H3 expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls). ( E–G ) Relative of western blot analysis of IGFBP-1, p-STAT3, and STAT3 expression in whole-cell lysates of CON and KO treated as in ( C ). The values are normalized to the GAPDH expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls).

Journal: eLife

Article Title: Gαq-PKD/PKCμ signal regulating the nuclear export of HDAC5 to induce the IκB expression and limit the NF-κB-mediated inflammatory response essential for early pregnancy

doi: 10.7554/eLife.83083

Figure Lengend Snippet: ( A, B ) Relative of western blot analysis of IκBα and p-IκBα expression in whole-cell lysates of CON and KO cells. The values are normalized to the GAPDH expression level and indicated as the mean ± standard error of the mean (SEM) of three independent experiments ( n = 3, *p < 0.05, versus controls). ( C, D ) Relative of western blot analysis of the p65 and p50 expression in cytoplasm and nucleus of CON and KO treated with lentivirus transfected with empty vector or NF κ BIA overexpression lentivirus for 3 days. The values are normalized to the GAPDH or H3 expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls). ( E–G ) Relative of western blot analysis of IGFBP-1, p-STAT3, and STAT3 expression in whole-cell lysates of CON and KO treated as in ( C ). The values are normalized to the GAPDH expression level and indicated as the mean ± SEM of three independent experiments ( n = 3, *p < 0.05, versus controls).

Techniques: Western Blot, Expressing, Transfection, Plasmid Preparation, Over Expression

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